Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone
Identifieur interne : 001E42 ( Main/Exploration ); précédent : 001E41; suivant : 001E43Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone
Auteurs : Qing-Hua He [République populaire de Chine] ; YANG XU [République populaire de Chine] ; Cun-Zheng Zhang [République populaire de Chine] ; Yan-Ping Li [République populaire de Chine] ; Zhi-Bing Huang [République populaire de Chine]Source :
- Food control [ 0956-7135 ] ; 2014.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The advantageous characteristics of phage probes and facility of immunoassays were combined to develop a rapid dot-immunoassay for the mycotoxin zearalenone (ZEN). A peptide library of random 12-mers displayed on phage was panned against anti-ZEN antibody. Selected phage-borne peptidomimetics were used as substitute for coating antigen and applied in dot-immunoassay for rapid detecting of ZEN. The binding specificities and reaction kinetics between selected phages and antibody were analyzed by phage ELISA and surface plasmon resonance, respectively. The equilibrium dissociation constant (KD) measured for selected phage (Z5): antibody was 39.8 nM. The cut-off level for this phage-based dot-immunoassay method of detecting ZEN in cereal samples, assessed visually, was 50 μg kg-1 and the final results can be obtained within 10 min. The validation of the method was performed by analyzing the spiked samples with ZEN at five levels (15, 30, 45, 60, and 75 μg kg-1) and naturally contaminated cereal samples, the results were in good agreement with the obtained by the commercial ZEN ELISA kit. These results suggest that phage can act as a useful immunochemical reagent in dot-immunoassay for toxic small molecules.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000023
- to stream PascalFrancis, to step Curation: 000071
- to stream PascalFrancis, to step Checkpoint: 000010
- to stream Main, to step Merge: 001E57
- to stream Main, to step Curation: 001E42
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone</title><author><name sortKey="He, Qing Hua" sort="He, Qing Hua" uniqKey="He Q" first="Qing-Hua" last="He">Qing-Hua He</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author><author><name sortKey="Yang Xu" sort="Yang Xu" uniqKey="Yang Xu" last="Yang Xu">YANG XU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author><author><name sortKey="Zhang, Cun Zheng" sort="Zhang, Cun Zheng" uniqKey="Zhang C" first="Cun-Zheng" last="Zhang">Cun-Zheng Zhang</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street</s1><s2>Nanjing 210014</s2><s3>CHN</s3><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Nanjing 210014</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yan Ping" sort="Li, Yan Ping" uniqKey="Li Y" first="Yan-Ping" last="Li">Yan-Ping Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author><author><name sortKey="Huang, Zhi Bing" sort="Huang, Zhi Bing" uniqKey="Huang Z" first="Zhi-Bing" last="Huang">Zhi-Bing Huang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">14-0094129</idno><date when="2014">2014</date><idno type="stanalyst">PASCAL 14-0094129 INIST</idno><idno type="RBID">Pascal:14-0094129</idno><idno type="wicri:Area/PascalFrancis/Corpus">000023</idno><idno type="wicri:Area/PascalFrancis/Curation">000071</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000010</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000010</idno><idno type="wicri:doubleKey">0956-7135:2014:He Q:phage:borne:peptidomimetics</idno><idno type="wicri:Area/Main/Merge">001E57</idno><idno type="wicri:Area/Main/Curation">001E42</idno><idno type="wicri:Area/Main/Exploration">001E42</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone</title><author><name sortKey="He, Qing Hua" sort="He, Qing Hua" uniqKey="He Q" first="Qing-Hua" last="He">Qing-Hua He</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author><author><name sortKey="Yang Xu" sort="Yang Xu" uniqKey="Yang Xu" last="Yang Xu">YANG XU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author><author><name sortKey="Zhang, Cun Zheng" sort="Zhang, Cun Zheng" uniqKey="Zhang C" first="Cun-Zheng" last="Zhang">Cun-Zheng Zhang</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street</s1><s2>Nanjing 210014</s2><s3>CHN</s3><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Nanjing 210014</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yan Ping" sort="Li, Yan Ping" uniqKey="Li Y" first="Yan-Ping" last="Li">Yan-Ping Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author><author><name sortKey="Huang, Zhi Bing" sort="Huang, Zhi Bing" uniqKey="Huang Z" first="Zhi-Bing" last="Huang">Zhi-Bing Huang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road</s1><s2>Nanchang 330047</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Nanchang</settlement><region type="province">Jiangxi</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Food control</title><title level="j" type="abbreviated">Food control</title><idno type="ISSN">0956-7135</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Food control</title><title level="j" type="abbreviated">Food control</title><idno type="ISSN">0956-7135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term><term>Immunological method</term><term>Mycotoxin</term><term>Peptides</term><term>Phage</term><term>Reagents</term><term>Zearalenone</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Réactif</term><term>Détection</term><term>Méthode immunologique</term><term>Mycotoxine</term><term>Zéaralénone</term><term>Bactériophage</term><term>Peptide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The advantageous characteristics of phage probes and facility of immunoassays were combined to develop a rapid dot-immunoassay for the mycotoxin zearalenone (ZEN). A peptide library of random 12-mers displayed on phage was panned against anti-ZEN antibody. Selected phage-borne peptidomimetics were used as substitute for coating antigen and applied in dot-immunoassay for rapid detecting of ZEN. The binding specificities and reaction kinetics between selected phages and antibody were analyzed by phage ELISA and surface plasmon resonance, respectively. The equilibrium dissociation constant (KD) measured for selected phage (Z5): antibody was 39.8 nM. The cut-off level for this phage-based dot-immunoassay method of detecting ZEN in cereal samples, assessed visually, was 50 μg kg<sup>-1</sup> and the final results can be obtained within 10 min. The validation of the method was performed by analyzing the spiked samples with ZEN at five levels (15, 30, 45, 60, and 75 μg kg<sup>-1</sup>) and naturally contaminated cereal samples, the results were in good agreement with the obtained by the commercial ZEN ELISA kit. These results suggest that phage can act as a useful immunochemical reagent in dot-immunoassay for toxic small molecules.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Jiangxi</li></region><settlement><li>Nanchang</li></settlement></list><tree><country name="République populaire de Chine"><region name="Jiangxi"><name sortKey="He, Qing Hua" sort="He, Qing Hua" uniqKey="He Q" first="Qing-Hua" last="He">Qing-Hua He</name></region><name sortKey="Huang, Zhi Bing" sort="Huang, Zhi Bing" uniqKey="Huang Z" first="Zhi-Bing" last="Huang">Zhi-Bing Huang</name><name sortKey="Li, Yan Ping" sort="Li, Yan Ping" uniqKey="Li Y" first="Yan-Ping" last="Li">Yan-Ping Li</name><name sortKey="Yang Xu" sort="Yang Xu" uniqKey="Yang Xu" last="Yang Xu">YANG XU</name><name sortKey="Zhang, Cun Zheng" sort="Zhang, Cun Zheng" uniqKey="Zhang C" first="Cun-Zheng" last="Zhang">Cun-Zheng Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E42 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E42 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:14-0094129
|texte= Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone
}}
| This area was generated with Dilib version V0.6.33. |  |